Ngwa nyocha ngwa ngwa syphilis (Chromatography nke azụ)

Nkọwa ngwaahịa:

Ezubere iji:

Ngwa nyocha ngwa ngwa syphilis (Lateral Chromatography) bụ ngwa ngwa immunoassay chromatographic maka nchọpụta qualitative nke ọgwụ mgbochi TP na ọbara dum, serum ma ọ bụ plasma iji nyere aka na nyocha nke syphilis.

Ụkpụrụ ule:

Ngwa nyocha ngwa ngwa syphilis dabere na nyocha nke immunochromatographic iji chọpụta ọgwụ mgbochi TP na ọbara dum, serum ma ọ bụ plasma.N'oge ule ahụ, ọgwụ mgbochi TP na-ejikọta ya na antigens TP nke akpọrọ na ihe ndị nwere agba agba ka ọ bụrụ mgbagwoju anya.N'ihi ọrụ capillary, mgbagwoju anya nke na-alụso ọrịa ọgụ na-agafe n'ime akpụkpọ ahụ.Ọ bụrụ na ihe nlele ahụ nwere ọgwụ mgbochi TP, a ga-ejide ya site na mpaghara ule ekpuchiburu ya wee mepụta ahịrị ule a na-ahụ anya.Iji jee ozi dị ka njikwa usoro, akara njikwa agba ga-apụta ma ọ bụrụ na emechara ule ahụ nke ọma

Isi ọdịnaya:

Maka Stripe:

Maka Cassette:

Usoro ọrụ

• Nzọụkwụ 1: Nkwadebe ihe atụ

Enwere ike ime ngwa nyocha ngwa ngwa syphilis (Lateral Chromatography) site na iji ọbara dum, serum ma ọ bụ plasma.

1. Kewapụ serum ma ọ bụ plasma na ọbara ozugbo enwere ike iji zere hemolysis.Jiri naanị ihe nlere anya na-abụghị hemolyzed doro anya.

2. Ekwesịrị ịme ule ozugbo ka achịkọtachara ihe nlele ahụ.Ọ bụrụ na enweghị ike ịmecha nnwale ozugbo, a ga-echekwa serum na plasma na 2-8 Celsius C ruo ụbọchị 3, maka nchekwa ogologo oge, a ga-echekwa ihe nlele na -20 ℃.Ekwesịrị ịchekwa ọbara niile anakọtara site na venipuncture na 2-8 Celsius ma ọ bụrụ na a ga-eme nnwale ahụ n'ime ụbọchị 2 nke nchịkọta.Akwụsịla ụdị ọbara dum.Ekwesịrị ịnwale ọbara niile anakọtara site na mkpịsị aka ozugbo.

3. A ghaghị iweghachite ihe nlele n'ime ụlọ okpomọkụ tupu anwale.A na-achọrọ ihe nlele oyi kpọnwụrụ ka agbaze kpamkpam wee gwakọta ya nke ọma tupu anwale ya, na-ezere ka oyi na-atụ ugboro ugboro na agbaze.

4. Ọ bụrụ na a ga-ebupu ihe nlere anya, a ga-akwakọba ha n'usoro iwu obodo na-ekpuchi njem nke ndị ọrụ etiologic.

• Nzọụkwụ 2: Nnwale

Kwe ka mpempe ule/kaseti, ihe nlere, ihe nlere anya ka ọ ruo n'ọnụ ụlọ

okpomọkụ (15-30°C) tupu anwale.

1. Wepu mpempe ule / cassette na obere akpa ahụ mechiri emechi ma jiri ya n'ime nkeji iri atọ.

2. Debe eriri ule/kaseti n'elu ala dị ọcha na larịị.

2.1 Maka ụdị ọbara ọbara ma ọ bụ Plasma:

Jide dropper ahụ kwụ ọtọ, dọpụta ihe nlele ahụ ruo n'ala Fill Line (ihe dị ka 40uL), wee bufee ihe nlele ahụ na nke ọma (S) nke eriri ule/cassette, wee tinye 1 drop of sample diluent (ihe dị ka 40uL) wee malite malite. ngụ oge.Zere ọnyà ikuku n'ime olulu mmiri (S).Lee ihe atụ n'okpuru.

2.2 Maka Ọbara Nile (Venipuncture/Fingerstick) Ụdị:

Jide dropper ahụ kwụ ọtọ, see ihe atụ ahụ gaa n'elu Fill Line (ihe dị ka 80uL), wee bufee ọbara dum na ụdị nke ọma (S) nke mpempe ule/cassette, wee tinye 1 drop of sample diluent (ihe dị ka 40uL) wee malite. ngụ oge.Zere ọnyà ikuku n'ime olulu mmiri (S).Lee ihe atụ n'okpuru.

• Nzọụkwụ 3: Ịgụ

3. Jiri anya gụọ nsonaazụ mgbe nkeji 10-20 gachara.Nsonaazụ abaghị uru ka nkeji iri abụọ gachara.

Ịkọwa nsonaazụ ya

1. Nsonaazụ dị mma

Ọ bụrụ na ma ahịrị C nke njikwa mma na ahịrị nchọpụta T pụtara, ọ na-egosi na ihe nlere ahụ nwere ọnụọgụ mgbochi TP a na-achọpụta, na nsonaazụ ya dị mma maka syphilis.

2. Nsonaazụ adịghị mma

Ọ bụrụ na naanị akara njikwa mma C na-egosi na nchọta T ahịrị anaghị egosi agba, ọ na-egosi na a naghị achọpụta ọgwụ mgbochi TP na ụdị.na nsonaazụ adịghị mma maka syphilis.

3. Nsonaazụ ezighi ezi

Ọ nweghị eriri nwere agba a na-ahụ anya na-egosi n'ahịrị njikwa ka emechara ule ahụ, nsonaazụ nnwale ezighi ezi.Megharịa nlele ahụ.

Ozi ịtụ: