Nkọwapụta Protein Cell Yeast
Usoro okwu yist bụ usoro a na-ejikarị eme ihe maka okwu protein eukaryotic, n'ihi ịdị mfe ya n'ịkọ ihe, oke ego, na ịdị mfe nke ọrụ.N'ime ụdị yist dị iche iche, Pichia pastoris bụ onye mgbasa ozi kachasị ewu ewu, ebe ọ na-eme ka okwu protein intracellular na extracellular dị mfe.Sistemu ahụ na-enyekwa aka mgbanwe mgbanwe ntụgharị, dị ka phosphorylation na glycosylation, na-ebute usoro okwu eukaryotic pụrụ iche nwere ọtụtụ uru.
| Ihe ọrụ | Oge ndu (BD) |
| Nkwalite nke Codon, njikọ mkpụrụ ndụ ihe nketa na subcloning | 5-10 |
| Nleba anya nke clone dị mma | 10-15 |
| Obere okwu okwu | |
| Nnukwu ọnụ ọgụgụ (200ML) nkwupụta na ịdị ọcha, ihe ndị a na-enweta na-agụnye protein dị ọcha na akụkọ nnwale |
Ọ bụrụ na a na-emepụta mkpụrụ ndụ ihe nketa na Bioantibody, a ga-etinye plasmid arụpụtara n'ime ihe ndị a na-ebupụta.