Ngwa Nnwale Ọrịa IgG/IgM nke Leishmania (Nnwale Immunochromatographic)

Nkọwa Dị Mkpirikpi:

Ihe nlele	Ọbara/Plasma/Ọbara dum	Usoro	Kaseti
Mmetụta uche	98.83%	Nkọwapụta	98.02%
Mgbanwe na Ọnọdụ Okpomọkụ.	2-30℃ / 36-86℉	Oge Ule	Nkeji 5-10
Nkọwapụta	1 Ule/Ngwa 5 Ule/Ngwa 25 Ule/Ngwa

Zitere anyị ozi-e

Nkọwa Ngwaahịa

Akara Ngwaahịa

Nkọwa ngwaahịa

Ojiji Ezubere
Ngwaahịa a dabara adaba maka nyocha ahụike nke serum/plasma/ihe nlele ọbara dum iji chọpụta ọgwụ mgbochi megide Leishmania. Ọ bụ nnwale dị mfe, ngwa ngwa na nke na-abụghị nke ngwa maka nchọpụta nke kala-azar nke Leishmania kpatara.

Ụkpụrụ Ule
Ngwaahịa a bụ ihe nyocha immunoassay nke na-eme n'akụkụ mmiri nke na-agbapụta n'akụkụ. Kaseti nnwale ahụ nwere: 1) ihe mkpuchi conjugate agba burgundy nke nwere recombinant rK39 antigen jikọtara ya na colloid gold (Leishmania conjugates) na rabbit IgG-gold conjugates; 2) ihe mkpuchi nitrocellulose nke nwere ihe mgbochi ule abụọ (M na G bands) na ihe njikwa (C band).

Isi ihe dị n'ime ya

E depụtara ihe ndị dị na tebụl ahụ.

Ihe / enyere	Ọnụọgụ (Nnwale/Ngwa 1)	Ọnụọgụ (Nnwale 5/Ngwa)	Ọnụọgụ (Nnwale 25/Ngwa)
Ngwa Nnwale	1 ule	Ule ise	Ule iri abụọ na ise
Nchekwa nchekwa	1 karama	Karama ise	Karama 25/2
Dọpa	1 ibe	Iberibe ise	Iberibe iri abụọ na ise
Akpa Njem Ihe Nlereanya	1 ibe	Iberibe ise	Iberibe iri abụọ na ise
Lancet a na-atụfu	1 ibe	Iberibe ise	Iberibe iri abụọ na ise
Ntuziaka maka Ojiji	1 ibe	1 ibe	1 ibe
Asambodo Nkwenye	1 ibe	1 ibe	1 ibe

Usoro Ọrụ

Nzọụkwụ 1: Ịtụle ihe nlele

Nakọta ọbara mmadụ/ọbara plasma/ọbara dum nke ọma.

Nzọụkwụ nke 2: Nnwale

Biko gụọ ntuziaka ndị a nke ọma tupu ị nwalee ha. Tupu ị nwalee ha, kwe ka ngwa nnwale ahụ, ihe ngwọta ihe nlele ahụ, na ihe nlele ahụ kwekọọ na okpomọkụ (15-30°C ma ọ bụ 59-86°Frehent).
1. Wepụ ọkpọkọ mwepụ na ngwa ahụ, tinyekwa igbe nnwale n'ime akpa ihe nkiri ahụ site n'ịdọwa oghere ahụ. Tinye ha n'elu oghere kwụ ọtọ.
2. Mepee akpa foil nke kaadị nlele ahụ. Wepụ kaadị nnwale ahụ wee tinye ya n'elu tebụl kwụ ọtọ.
3. Jiri paịpụt a na-atụfu atụfu, tinye otu ntapụ (ihe dị ka 20μL) ọbara/ma ọ bụ serum 4μL/ma ọ bụ plasma 4μL/ ma ọ bụ 4μL ọbara dum n'ime olulu mmiri dị na kaseti nnwale ahụ.
4. Mepee ọkpọkọ nchekwa ahụ. Tinye ntapụ atọ (ihe dị ka 80 μL) nke ihe na-agbaze n'ime ihe na-agbaze n'ime ihe na-agbaze n'ime ihe na-agbaze n'ime nke ọma. Malite ịgụta.

Nzọụkwụ nke 3: Ịgụ Ihe

Gụọ nsonaazụ ya n'ime nkeji 5-10. Nsonaazụ mgbe nkeji 10 gachara adịghị mma.

Nkọwa Nsonaazụ

Nsonaazụ na-adịghị mma
Ọ bụrụ naanị akara njikwa mma C pụtara na ahịrị nchọpụta G na M egosighi

Nsonaazụ Dị Mma
1. Ma ahịrị njikwa mma C na ahịrị nchọpụta M pụtara = achọpụtara ihe mgbochi Leishmania IgM, nsonaazụ ya dịkwa mma maka ihe mgbochi IgM.
2. Ma ahịrị njikwa mma C na ahịrị nchọpụta G pụtara = achọpụtara ihe mgbochi Leishmania IgG na nsonaazụ ya dị mma maka mgbochi IgG.
3. A na-ahụ ma akara njikwa mma C na ahịrị nchọpụta G na M = a na-achọpụta ọgwụ mgbochi IgG na IgM Leishmania, nsonaazụ ya dịkwa mma maka ma ọgwụ mgbochi IgG na IgM.

Nsonaazụ na-ezighi ezi
Agaghị ahụ akara njikwa àgwà C, nsonaazụ agaghị adị irè n'agbanyeghị ma akara nnwale ahụ ọ pụtara, a ga-emekwa ule ahụ ọzọ.

Ozi Ịtụ Ihe

Aha Ngwaahịa	Nwamba. Mba	Nha	Ihe nlele	Ndụ nchekwa	Mgbanwe na Ọnọdụ Okpomọkụ.
Ngwa Nnwale Ọrịa IgG/IgM nke Leishmania (Nnwale Immunochromatographic)	B020C-01	1nwale/ngwaọrụ	Ọbara/Plasma/Ọbara dum	Ọnwa iri na asatọ	2-30℃ / 36-86℉
	B020C-05	Nnwale/ngwa 5
	B020C-25	Nnwale 25/ngwa