Ngwa ule Leishmania IgG/IgM (Immunochromatographic assay) onyonyo egosipụtara

Ngwa nyocha nke Leishmania IgG/IgM (Immunochromatographic assay)

Nkọwa dị mkpirikpi:

Ụdị	Ọbara/Plasma/Ọbara Nile	Usoro	Cassette
Mmetụta	98.83%	Nkọwapụta	98.02%
Trans.& Sto.Okpomọkụ	2-30℃ / 36-86℉	Oge ule	5-10 nkeji
Nkọwapụta	1 Test/Kit 5 Test/Kit 25 Test/Kit

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwa ngwaahịa

Ezubere iji
Ngwaahịa a dabara adaba maka nyocha ụlọ ọgwụ qualitative nke serum/plasma/ihe nlele ọbara niile maka ịchọpụta ọgwụ mgbochi megide Leishmania.Ọ bụ ule dị mfe, ngwa ngwa na anaghị arụ ọrụ maka nyocha nke kala-azar nke Leishmania kpatara.

Ụkpụrụ ule
Ngwaahịa a bụ immunoassay chromatographic mpụta.Kaseti ule ahụ nwere: 1) pad conjugate nwere agba burgundy nwere recombinant rK39 antigen conjugated na colloid gold (Leishmania conjugates) na oke bekee IgG-ọlaedo conjugates;2) eriri akpụkpọ anụ nitrocellulose nwere eriri ule abụọ (M na G band) na otu njikwa (C band).

Isi ọdịnaya

Edepụtara ihe ndị enyere na tebụl.

Akụrụngwa / enyere	Ọnụọgụ (1 Nnwale/Kit)	Ọnụọgụ(Nnwale 5/Kit)	Ọnụọgụ(Nnwale 25/Kit)
Ngwa ule	1 ule	5 ule	25 ule
Ihe nchekwa	1 karama	karama 5	25/2 karama
Dropper	1 ibe	5 pcs	25pcs
Akpa njem ihe atụ	1 ibe	5 pcs	25pcs
Lancet enwere ike iwepụ	1 ibe	5 pcs	25pcs
Ntuziaka maka ojiji	1 ibe	1 ibe	1 ibe
Asambodo Nkwekọrịta	1 ibe	1 ibe	1 ibe

Usoro ọrụ

Nzọụkwụ 1: Nlereanya

Chịkọta Serum/Plasma/Ọbara dum nke ọma.

Nzọụkwụ 2: Nnwale

Biko gụọ ntuziaka ahụ nke ọma tupu nnwale.Tupu anwale, kwe ka ngwa ule, ihe nlere anya na ihe nlele wee guzozie na okpomọkụ (15-30℃ ma ọ bụ 59-86 degrees Fahrenheit).
1.Wepu tube mmịpụta site na kit na igbe nyocha site na akpa ihe nkiri site na ịdọwa notch.Tinye ha n'ụgbọ elu kwụ ọtọ.
2.Mepee kaadị nyocha nke aluminum foil bag.Wepu kaadị ule ma tinye ya n'ahịrị na tebụl.
3.Jiri pipette mkpofu, nyefee otu dobe (ihe dị ka 20μL) ọbara mkpịsị aka / ma ọ bụ 4μL serum / ma ọ bụ 4μL plasma/ ma ọ bụ 4μL ọbara dum n'ime ihe nlele nke ọma na cassette ule.
4.Mepee tube buffer.Tinye 3 tụlee (ihe dị ka 80 μL) nke assay diluent n'ime ihe na-eme nchọpụta nke ọma nwere ọdịdị okirikiri.Malite ịgụta ọnụ.

Nzọụkwụ 3: Ịgụ

Gụọ nsonaazụ ya na nkeji 5-10.Nsonaazụ ka nkeji iri gachara abaghị uru.

Nkọwapụta nsonaazụ

Nsonaazụ adịghị mma
Ọ bụrụ na naanị ahịrị njikwa mma C pụtara na ahịrị nchọpụta G na M anaghị egosi

Nsonaazụ dị mma
1.Ma akara njikwa mma C na akara nchọpụta M pụtara = a na-achọpụta ihe mgbochi Leishmania IgM, nsonaazụ ya dị mma maka mgbochi IgM.
2.Ma akara njikwa mma C na akara nchọpụta G pụtara = a na-achọpụta ihe mgbochi Leishmania IgG na nsonaazụ ya dị mma maka mgbochi IgG.
3.Ma ahịrị njikwa mma C na ahịrị nchọpụta G na M pụtara = ọgwụ mgbochi Leishmania IgG na IgM.Achọpụtara ya, nsonaazụ ya dịkwa mma maka ọgwụ mgbochi IgG na IgM.

Nsonaazụ ezighi ezi
Enweghị ike ịhụ ahịrị njikwa mma C, nsonaazụ agaghị adị irè n'agbanyeghị ma ahịrị ule gosipụtara, ma ekwesịrị ịmegharị ule ahụ ọzọ.

Ozi ịtụ

Aha ngwaahịa	Nwamba.Mba	Nha	Ụdị	Ndụ shelf	Trans.& Sto.Okpomọkụ
Ngwa nyocha nke Leishmania IgG/IgM (Immunochromatographic assay)	B020C-01	1 ule/kit	Ọbara/Plasma/Ọbara Nile	Ọnwa 18	2-30℃ / 36-86℉
	B020C-05	5 ule/kit
	B020C-25	25 ule/kit