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Ngwa ule ngwa ngwa nke obi troponin (Chromatography n'azụ)

Nkọwa dị mkpirikpi:

Ụdị

Ọbara/Plasma/Ọbara Nile

Usoro

Cassette

Mmetụta

99.60%

Nkọwapụta

98.08%

Trans.& Sto.Okpomọkụ

2-30℃ / 36-86℉

Oge ule

10-30 nkeji

Nkọwapụta

1 Nnwale/Kit;25 Nnwale/Kit


Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwa ngwaahịa

Ezubere iji:

Ngwa ule ngwa ngwa nke Cardiac Troponin I na-etinye immunochromatography ọla edo colloidal iji chọpụta Troponin I (cTnI) obi na serum, plasma ma ọ bụ ihe nlele ọbara dum n'ụzọ qualitatively ma ọ bụ ọkara nke nwere kaadị colorimetric ọkọlọtọ.A na-eji ule a dị ka ihe enyemaka n'ịchọpụta mmerụ ahụ nke myocardial dị ka Myocardial Infarction, Unstable Angina, Acute Myocarditis and Acute Coronary Syndrome.

Ụkpụrụ ule:

Ngwa ule ngwa ngwa nke Cardiac Troponin I (Lateral Chromatography) bụ qualitative ma ọ bụ ọkara quntitative, akpụkpọ ahụ dabeere na immunoassay maka nchọpụta nke obi Troponin I (cTnI) n'ime ọbara dum, serum ma ọ bụ plasma.N'ime usoro nnwale a, a na-ewepụ reagent njide na mpaghara akara ule nke ule ahụ.Mgbe agbakwunyere ihe nlere n'akụkụ ihe atụ nke cassette ahụ, ọ na-emeghachi omume na ihe mgbochi cTnI mgbochi mmadụ n'ule.Ngwakọta a na-akwaga chromatographically n'ogologo ule ahụ wee na-emekọrịta ihe na reagent na-enweghị ike ijide.Ụdị ule ahụ nwere ike ịchọpụta Troponin I(cTnI) obi n'ụdị.Ọ bụrụ na ihe atụ ahụ nwere obi Troponin I (cTnI), eriri agba ga-apụta na mpaghara akara ule na agba agba nke akara ule na-abawanye n'ogo nke cTnI, na-egosi nsonaazụ dị mma.Ọ bụrụ na ihe nlere ahụ enweghị obi Troponin I(cTnI), eriri agba agaghị apụta na mpaghara a, na-egosi nsonaazụ na-adịghị mma.Iji jee ozi dị ka njikwa usoro, eriri agba ga-apụta mgbe niile na mpaghara ahịrị akara, na-egosi na agbakwunyere olu kwesịrị ekwesị nke ihe nlere anya na akpụkpọ anụ ahụ emeela.

Isi ọdịnaya

Edepụtara ihe ndị enyere na tebụl.

Akụkụ REF

REF

B032C-01

B032C-25

Nwalee cassette

1 ule

25 ule

Sample diluent

1 karama

1 karama

Dropper

1 ibe

25pcs

Kaadị colorimetric ọkọlọtọ

1 ibe

1 ibe

Asambodo Nkwekọrịta

1 ibe

1 ibe

Usoro ọrụ

Nzọụkwụ 1: Nkwadebe ihe atụ

1. Enwere ike ime ngwa nyocha site na iji ọbara dum, serum ma ọ bụ plasma.Na-atụ aro ịhọrọ serum ma ọ bụ plasma dị ka nlele ule.Ọ bụrụ na họrọ ọbara zuru oke dị ka nlele ule, a ga-eji ya na mgbaze ihe nlele ọbara.

2. Nyochaa ihe nlele na kaadị ule ozugbo.Ọ bụrụ na enweghi ike ịmecha nyocha ozugbo, a ga-echekwa ọbara ọbara na plasma ruo ụbọchị 7 na 2 ~ 8 ℃ ma ọ bụ chekwaa na -20 ℃ maka ọnwa 6 (n'ime ọbara niile kwesịrị ịchekwa ruo ụbọchị 3 na 2 ~ 8 ℃). ) ruo mgbe a ga-anwale ya.

3. A ghaghị iweghachite ihe nlele n'ime ụlọ okpomọkụ tupu anwale.A na-achọrọ ihe nlele oyi kpọnwụrụ ka agbaze kpamkpam wee gwakọta ya nke ọma tupu anwale ya, na-ezere ka oyi na-atụ ugboro ugboro na agbaze.

4. Zere ikpo ọkụ samples, nke nwere ike ime ka hemolysis na protein denaturation.A na-atụ aro ka ị zere iji ihe nlele hemolyzed siri ike.Ọ bụrụ na ihe nlele yiri ka ọ na-emecha ọbara ọbara nke ukwuu, a ga-enweta ihe nlele ọzọ wee nwalee ya.

Nzọụkwụ 2: Nnwale

1. Biko gụọ akwụkwọ ntuziaka ahụ nke ọma tupu ịnwale, weghachi ihe nlele, kaadị ule na ihe nlele ọbara na okpomọkụ ụlọ wee gụọ kaadị ahụ.Na-atụ aro imeghe akpa foil ka ọ gbakere n'ime ụlọ ma jiri kaadị ule ozugbo.

2. Tinye kaadị ule na tebụl dị ọcha, tinye ya n'ahịrị.

Maka nyocha ọbara ma ọ bụ Plasma:

Jide dropper ahụ kwụ ọtọ ma bufee mmiri mmiri 3 nke serum ma ọ bụ plasma (ihe dị ka 80 L, enwere ike iji Pipette mee ihe na mberede) n'ụdị nke ọma, wee malite ngụ oge.Lee ihe atụ n'okpuru.

Plasma ụdị 1

Maka Ụdị Ọbara Nile:

Jide dropper ahụ kwụ ọtọ ma bufee 3 nke ọbara dum (ihe dị ka 80 L) n'ụdị nke ọma, tinyezie 1 dobe nke ihe nrịbama Sample (ihe dị ka 40 L), wee malite ngụ oge.Lee ihe atụ n'okpuru.

Plasma ụdị 2

Nzọụkwụ 3: Ịgụ

N'ime nkeji 10-30, nweta nsonaazụ nke ọkara dị ka kaadị colorimetric ọkọlọtọ site na anya.

Ịkọwa nsonaazụ ya

Plasma ụdị 3

Kwesịrị ekwesị: Otu eriri uhie na-acha odo odo na-egosi na akara njikwa (C).Maka nsonaazụ ziri ezi, ị nwere ike nweta ọkara-quantitative site na anya na kaadị colorimetric ọkọlọtọ:

Agba agba vs ntụnye ntụaka

Agba ike

Ntụtụ aka (ng/ml)

-

.0.5

+ -

0,5-1

+

1 ~ 5

+ +

5-15

+ + +

15-30

+ + + +

30-50

+ + + +

50

Na adịghị mma: Ọ dịghị purplish red streak pụtara na akara akara (C) .Nke a pụtara na ụfọdụ arụmọrụ ga-ezighị ezi ma ọ bụ ule kaadị adịlarị irè.N'ọnọdụ a, biko gụọ akwụkwọ ntuziaka ahụ nke ọma ọzọ, ma gbalịa ọzọ na cassette ọhụrụ. Ọ bụrụ na otu ọnọdụ ahụ emee ọzọ, ị kwesịrị ịkwụsị iji ngwaahịa a ozugbo wee kpọtụrụ onye na-eweta gị.

Ozi ịtụ

Aha ngwaahịa

Nwamba.Mba

Nha

Ụdị

Ndụ shelf

Trans.& Sto.Okpomọkụ

Ngwa ule ngwa ngwa nke obi troponin (Chromatography n'azụ)

B032C-01

1 ule/kit

S/P/WB

Ọnwa 24

2-30 ℃

B032C-25

25 ule/kit


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