Ọrụ mgbochi ọrịa monoclonal
Mgbochi Monoclonal, nke otu mkpụrụ ndụ B cell mepụtara, nwere oke nkwekọ nke na-elekwasị anya na epitope antigen.Ọgwụ mgbochi Monoclonal nwere ọtụtụ uru, gụnyere ịdị ọcha dị elu, uche na nkọwapụta.A na-emepụta hybridomas site na njikọta nke lymphocytes B nke na-emepụta ụfọdụ ọgwụ mgbochi ọrịa, nwere mkpụrụ ndụ myeloma dị ogologo ndụ.Bioantibody na-eji teknụzụ ngwakọta na-arụ ọrụ nke ọma nke ji okpukpu 20 dị irè karịa ụzọ njikọta oge ochie.Na mgbakwunye, ọ na-eji teknụzụ nyocha microarray protein iji chọpụta ọgwụ mgbochi monoclonal na-egosipụta nkọwapụta dị elu, mmekọ, yana arụmọrụ arụ ọrụ megide epitopes akọwapụtara.
| Ihe ọrụ | Ọdịnaya nnwale | Oge ndu (izu) |
| Nkwadebe Antigen | 1. Onye ahịa na-enye antigen2. Bioantibody na-akwadebe antigen | / |
| Mgbochi òké | Ịgba ọgwụ mgbochi BALB/c òké, nchịkọta serum na nyocha ELISA | 4 |
| Ngwakọta cell na nyocha | Ngwakọta splenocytes òké na sel myeloma, nyocha okpu | 2 |
| Nrụpụta eriri cell kwụsiri ike | Mwepu nke clones dị mma enyo enyo | 3 |
| Isotype njirimara mgbochi | Nchọpụta subtypes ahịrị cell | 1 |
| Mkpokọta ọnụ ọgụgụ dị nta | Mmụba na-enweghị serum | 2 |
| Nnukwu ọnụ ọgụgụ incubation na ọcha | 200ml incubation na-adịghị ọcha serum | 1 |
